Development and validation of green chemistry HPLC-RI method for the simultaneous estimation of glucosamine and chondroitin sulfate from drug products

Design and development of analytical methods with environment friendly reagents solvents is the need hour for labs engaged in services. Every year thousands chemical pharmaceutical laboratories worldwide are generating tons toxic wastage causing environmental pollution thereby endangering human existence. The objective this study was to develop validate a sensitive, stability-indicating, accurate precise green chemistry RP-HPLC method RI detector simultaneous quantitation glucosamine chondroitin sulfate from various dosage forms. Complete separation both actives achieved isocratic mode by using Hypersil BDS Phenyl (250 x 4.6 mm, 5µm) HPLC column. Purified water as diluent phosphoric acid buffer pH 2.5 used mobile phase at flow rate 0.2 mL/min. column temperature maintained 40°C. Both molecules being non-chromophoric nature, refractive index (RI) detection. proposed does not require organic solvents, gradient elution complex derivatization unlike reported methods. developed successfully validated per ICH guidelines. stability indicating, sensitive economical. Hence, it can be routine analysis commercial batches these combination products.